cd44 im7 8 1 Search Results


mouse monoclonal anti cd44 antibody  (Cedarlane)
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Cedarlane mouse monoclonal anti cd44 antibody
Mouse Monoclonal Anti Cd44 Antibody, supplied by Cedarlane, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 1 article reviews
mouse monoclonal anti cd44 antibody - by Bioz Stars, 2026-10
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fitc conjugated rat anti mouse cd44  (Cedarlane)
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Cedarlane fitc conjugated rat anti mouse cd44
BCG infectious dose correlates with increased expression of the T-cell activation markers <t>CD44,</t> IL-2Rα, and IL-2Rβ. Unfractionated splenocytes (5 × 105 cells/tube) from each type of spleen were stained with <t>FITC-labeled</t> anti-mouse <t>CD44,</t> anti-mouse CD25/IL-2Rα, or anti-mouse CD122/IL-2Rβ and PE-labeled anti-mouse CD4 or anti-mouse CD8α. The percentages of CD4+ CD44hi, IL-2Rαhi, or IL-2Rβhi CD4+ (white bars) or CD8+ cells (black bars) in the spleen were determined by flow cytometry. Means ± standard errors of the means of three experiments are shown. ∗, P < 0.05; ∗∗, P < 0.01. Purified CD4+ T cells from the spleens of BCG HD-infected mice (day 16) were stained with FITC anti-mouse <t>CD44</t> and were sorted in an EPICS Elite ESP (Beckman Coulter) into CD44low (naïve) and CD44hi (memory/effector) populations. Sorted cells were cocultured at 105 cells/well either with bone marrow-derived dendritic cells (5 × 104 cells/well) in the presence or absence of SEB (10 μg/ml) or with M12 B-lymphoma cells (5 × 104 cells/well) in the presence or absence of BCG-Ag (10 μg/ml). IFN-γ levels were determined as described in Materials and Methods. Mean ± standard deviation of triplicate wells is shown.
Fitc Conjugated Rat Anti Mouse Cd44, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd44+im7+8+1/Anti-Mouse+CD44+%2C+FITC+(Clone+IM7%2E8%2E1)+(rat+IgG2b)/pmc00128208-145-60-65
Average 85 stars, based on 1 article reviews
fitc conjugated rat anti mouse cd44 - by Bioz Stars, 2026-10
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mouse anti rat cd44 igg2a cedarlane cl044  (Cedarlane)
85
Cedarlane mouse anti rat cd44 igg2a cedarlane cl044
Primary Antibodies Used in This Study
Mouse Anti Rat Cd44 Igg2a Cedarlane Cl044, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd44+im7+8+1/Anti-Mouse+CD44+%2C+Purified+(Clone+IM7%2E8%2E1)+(rat+IgG2b)/pmc04278240-162-173-177
Average 85 stars, based on 1 article reviews
mouse anti rat cd44 igg2a cedarlane cl044 - by Bioz Stars, 2026-10
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anti cd44 im7 8 1  (Cedarlane)
85
Cedarlane anti cd44 im7 8 1
Primary Antibodies Used in This Study
Anti Cd44 Im7 8 1, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd44+im7+8+1/Anti-Mouse+CD44+%2C+Biotin+(Clone+IM7%2E8%2E1)+(rat+IgG2b)/pm17913542-38-25-27
Average 85 stars, based on 1 article reviews
anti cd44 im7 8 1 - by Bioz Stars, 2026-10
85/100 stars
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Image Search Results


BCG infectious dose correlates with increased expression of the T-cell activation markers CD44, IL-2Rα, and IL-2Rβ. Unfractionated splenocytes (5 × 105 cells/tube) from each type of spleen were stained with FITC-labeled anti-mouse CD44, anti-mouse CD25/IL-2Rα, or anti-mouse CD122/IL-2Rβ and PE-labeled anti-mouse CD4 or anti-mouse CD8α. The percentages of CD4+ CD44hi, IL-2Rαhi, or IL-2Rβhi CD4+ (white bars) or CD8+ cells (black bars) in the spleen were determined by flow cytometry. Means ± standard errors of the means of three experiments are shown. ∗, P < 0.05; ∗∗, P < 0.01. Purified CD4+ T cells from the spleens of BCG HD-infected mice (day 16) were stained with FITC anti-mouse CD44 and were sorted in an EPICS Elite ESP (Beckman Coulter) into CD44low (naïve) and CD44hi (memory/effector) populations. Sorted cells were cocultured at 105 cells/well either with bone marrow-derived dendritic cells (5 × 104 cells/well) in the presence or absence of SEB (10 μg/ml) or with M12 B-lymphoma cells (5 × 104 cells/well) in the presence or absence of BCG-Ag (10 μg/ml). IFN-γ levels were determined as described in Materials and Methods. Mean ± standard deviation of triplicate wells is shown.

Journal:

Article Title: Mycobacterium bovis BCG-Infected Mice Are More Susceptible to Staphylococcal Enterotoxin B-Mediated Toxic Shock than Uninfected Mice despite Reduced In Vitro Splenocyte Responses to Superantigens †

doi: 10.1128/IAI.70.8.4148-4157.2002

Figure Lengend Snippet: BCG infectious dose correlates with increased expression of the T-cell activation markers CD44, IL-2Rα, and IL-2Rβ. Unfractionated splenocytes (5 × 105 cells/tube) from each type of spleen were stained with FITC-labeled anti-mouse CD44, anti-mouse CD25/IL-2Rα, or anti-mouse CD122/IL-2Rβ and PE-labeled anti-mouse CD4 or anti-mouse CD8α. The percentages of CD4+ CD44hi, IL-2Rαhi, or IL-2Rβhi CD4+ (white bars) or CD8+ cells (black bars) in the spleen were determined by flow cytometry. Means ± standard errors of the means of three experiments are shown. ∗, P < 0.05; ∗∗, P < 0.01. Purified CD4+ T cells from the spleens of BCG HD-infected mice (day 16) were stained with FITC anti-mouse CD44 and were sorted in an EPICS Elite ESP (Beckman Coulter) into CD44low (naïve) and CD44hi (memory/effector) populations. Sorted cells were cocultured at 105 cells/well either with bone marrow-derived dendritic cells (5 × 104 cells/well) in the presence or absence of SEB (10 μg/ml) or with M12 B-lymphoma cells (5 × 104 cells/well) in the presence or absence of BCG-Ag (10 μg/ml). IFN-γ levels were determined as described in Materials and Methods. Mean ± standard deviation of triplicate wells is shown.

Article Snippet: For flow cytometry analysis, splenocytes (5 × 10 5 ) were stained with all or some of the following MAbs (all at 1:100 dilutions in R10A): fluorescein isothiocyanate (FITC)-conjugated mouse anti-mouse Vβ8 TCR (F23.1), FITC-conjugated rat anti-mouse CD25 (IL-2 Rα; 7D4), FITC-conjugated rat anti-mouse CD122 (IL-2 Rβ; TM-β1), FITC-conjugated rat anti-mouse I-A d /I-E d (2G9) (all from PharMingen), or FITC-conjugated rat anti-mouse CD44 (IM7.8.1; Cedarlane Laboratories, Hornby, Canada).

Techniques: Expressing, Activation Assay, Staining, Labeling, Flow Cytometry, Purification, Infection, Derivative Assay, Standard Deviation

Primary Antibodies Used in This Study

Journal: The American Journal of Pathology

Article Title: Identification and Characterization of Mesenchymal-Epithelial Progenitor-Like Cells in Normal and Injured Rat Liver

doi: 10.1016/j.ajpath.2014.08.029

Figure Lengend Snippet: Primary Antibodies Used in This Study

Article Snippet: All fluorescent secondary antibodies were purchased from Jackson Immunoresearch Laboratories (West Grove, PA) and used at a dilution of 1:200: anti-mouse IgG (DyLight 549), number 715-505-151, subclass 1; anti-mouse IgG (cy2 conjugated, number 115-225-205), subclass 1; anti-mouse IgG (cy3 conjugated, number 115-165-206), subclass 2a; and anti-rabbit IgG (cy2 conjugated, number 715-165-151, and DyLight 488, number 715-095-151). table ft1 table-wrap mode="anchored" t5 caption a7 Antibodies Isotype Company or producer Catalog no. Dilution Mouse anti-KRT18 IgG1 Abcam (Cambridge, MA) Ab668 1:200 Rabbit anti-KRT18 Polyclonal Novus Biologicals, LLC (Littleton, CO) NBP1-67610 1:150 Rabbit anti-human CLDN 7 Polyclonal Abcam Ab27487 1:100 Rabbit anti-GFP Polyclonal Novus Biologicals, LLC NB-600-308 1:1000 Rabbit anti-rat Desmin Polyclonal AnaSpec, Inc. (San Jose, CA) 29593 1:200 Mouse anti-rat THY1/CD90 IgG1 Cedarlane (Hornby, ON, Canada) CL005AP 1:50 Mouse anti-rat THY1/CD90 IgG1 BD Biosciences (San Jose, CA) 553016 1:50 Mouse anti-rat THY1/CD90 IgG2a Antibody Solutions (Sunnyvale, CA) Ab53226 1:25 Mouse anti-CDH1 IgG2a Transduction Lab, BD Biosciences 61081 1:50 Mouse anti-CDH1 IgG1 ECM Biosciences (Versailles, KY) CM1681 1:50 Rabbit anti-GFP Polyclonal Novus Biologicals, LLC AB-4 1:500 Mouse anti-rat CD44 IgG2a Cedarlane CL044 1:50 Mouse OV6 IgG1 Kind gift from Dr. Stewart Sell (Wadsworth Center, Albany, NY) NA 1:50 Mouse anti-ACTA2 IgG2a Abcam Ab11003 1:100 Mouse anti-GFAP IgG1 Abcam Ab80842 1:400 Mouse anti-rat VCAM1 IgG1 Bio Legend (San Diego, CA) 200401 1:100 Rabbit anti-CD29/integrin B1 Monoclonal Epitomics (Burlingame, CA) 2288-1 1:50 Mouse anti-vimentin IgG1 Lab Vision Corporation (Fremont, CA) MS129-PO 1:50 Mouse anti-rat NT5E IgG1 BD Biosciences 551123 1:50 Open in a separate window NA, not applicable.

Techniques: Transduction

Primers Used in This Study

Journal: The American Journal of Pathology

Article Title: Identification and Characterization of Mesenchymal-Epithelial Progenitor-Like Cells in Normal and Injured Rat Liver

doi: 10.1016/j.ajpath.2014.08.029

Figure Lengend Snippet: Primers Used in This Study

Article Snippet: All fluorescent secondary antibodies were purchased from Jackson Immunoresearch Laboratories (West Grove, PA) and used at a dilution of 1:200: anti-mouse IgG (DyLight 549), number 715-505-151, subclass 1; anti-mouse IgG (cy2 conjugated, number 115-225-205), subclass 1; anti-mouse IgG (cy3 conjugated, number 115-165-206), subclass 2a; and anti-rabbit IgG (cy2 conjugated, number 715-165-151, and DyLight 488, number 715-095-151). table ft1 table-wrap mode="anchored" t5 caption a7 Antibodies Isotype Company or producer Catalog no. Dilution Mouse anti-KRT18 IgG1 Abcam (Cambridge, MA) Ab668 1:200 Rabbit anti-KRT18 Polyclonal Novus Biologicals, LLC (Littleton, CO) NBP1-67610 1:150 Rabbit anti-human CLDN 7 Polyclonal Abcam Ab27487 1:100 Rabbit anti-GFP Polyclonal Novus Biologicals, LLC NB-600-308 1:1000 Rabbit anti-rat Desmin Polyclonal AnaSpec, Inc. (San Jose, CA) 29593 1:200 Mouse anti-rat THY1/CD90 IgG1 Cedarlane (Hornby, ON, Canada) CL005AP 1:50 Mouse anti-rat THY1/CD90 IgG1 BD Biosciences (San Jose, CA) 553016 1:50 Mouse anti-rat THY1/CD90 IgG2a Antibody Solutions (Sunnyvale, CA) Ab53226 1:25 Mouse anti-CDH1 IgG2a Transduction Lab, BD Biosciences 61081 1:50 Mouse anti-CDH1 IgG1 ECM Biosciences (Versailles, KY) CM1681 1:50 Rabbit anti-GFP Polyclonal Novus Biologicals, LLC AB-4 1:500 Mouse anti-rat CD44 IgG2a Cedarlane CL044 1:50 Mouse OV6 IgG1 Kind gift from Dr. Stewart Sell (Wadsworth Center, Albany, NY) NA 1:50 Mouse anti-ACTA2 IgG2a Abcam Ab11003 1:100 Mouse anti-GFAP IgG1 Abcam Ab80842 1:400 Mouse anti-rat VCAM1 IgG1 Bio Legend (San Diego, CA) 200401 1:100 Rabbit anti-CD29/integrin B1 Monoclonal Epitomics (Burlingame, CA) 2288-1 1:50 Mouse anti-vimentin IgG1 Lab Vision Corporation (Fremont, CA) MS129-PO 1:50 Mouse anti-rat NT5E IgG1 BD Biosciences 551123 1:50 Open in a separate window NA, not applicable.

Techniques: Sequencing, Amplification

Immunofluorescence microscopy analysis for expression of mesenchymal and epithelial cell marker proteins in 13-11-3-2 cell line. Mesenchymal cell markers were as follows: vascular cell adhesion molecule 1 (VCAM; A); Desmin (B); ACTA2 (α smooth muscle actin; C); glial fibrillary acidic protein (GFAP; F); Vimentin (G); Thy1 (CD90; H); CD73 (Ecto-5′-Nucleotidase; K); and CD29 (Integrin β1; L); Epithelial cell markers were as follows: KRT18 (Cytokeratin 18; D); OV-6 (E); CD44 (I); CDH1 (epithelial cadherin; J); CLDN7 (Claudin-7; M); phase-contrast microphotograph of cultured 13-11-3-5 cells (N); and phase-contrast microphotograph of cultured 13-11-3-3 cells (O). Original magnifications: ×40 (M); ×10 (N and O).

Journal: The American Journal of Pathology

Article Title: Identification and Characterization of Mesenchymal-Epithelial Progenitor-Like Cells in Normal and Injured Rat Liver

doi: 10.1016/j.ajpath.2014.08.029

Figure Lengend Snippet: Immunofluorescence microscopy analysis for expression of mesenchymal and epithelial cell marker proteins in 13-11-3-2 cell line. Mesenchymal cell markers were as follows: vascular cell adhesion molecule 1 (VCAM; A); Desmin (B); ACTA2 (α smooth muscle actin; C); glial fibrillary acidic protein (GFAP; F); Vimentin (G); Thy1 (CD90; H); CD73 (Ecto-5′-Nucleotidase; K); and CD29 (Integrin β1; L); Epithelial cell markers were as follows: KRT18 (Cytokeratin 18; D); OV-6 (E); CD44 (I); CDH1 (epithelial cadherin; J); CLDN7 (Claudin-7; M); phase-contrast microphotograph of cultured 13-11-3-5 cells (N); and phase-contrast microphotograph of cultured 13-11-3-3 cells (O). Original magnifications: ×40 (M); ×10 (N and O).

Article Snippet: All fluorescent secondary antibodies were purchased from Jackson Immunoresearch Laboratories (West Grove, PA) and used at a dilution of 1:200: anti-mouse IgG (DyLight 549), number 715-505-151, subclass 1; anti-mouse IgG (cy2 conjugated, number 115-225-205), subclass 1; anti-mouse IgG (cy3 conjugated, number 115-165-206), subclass 2a; and anti-rabbit IgG (cy2 conjugated, number 715-165-151, and DyLight 488, number 715-095-151). table ft1 table-wrap mode="anchored" t5 caption a7 Antibodies Isotype Company or producer Catalog no. Dilution Mouse anti-KRT18 IgG1 Abcam (Cambridge, MA) Ab668 1:200 Rabbit anti-KRT18 Polyclonal Novus Biologicals, LLC (Littleton, CO) NBP1-67610 1:150 Rabbit anti-human CLDN 7 Polyclonal Abcam Ab27487 1:100 Rabbit anti-GFP Polyclonal Novus Biologicals, LLC NB-600-308 1:1000 Rabbit anti-rat Desmin Polyclonal AnaSpec, Inc. (San Jose, CA) 29593 1:200 Mouse anti-rat THY1/CD90 IgG1 Cedarlane (Hornby, ON, Canada) CL005AP 1:50 Mouse anti-rat THY1/CD90 IgG1 BD Biosciences (San Jose, CA) 553016 1:50 Mouse anti-rat THY1/CD90 IgG2a Antibody Solutions (Sunnyvale, CA) Ab53226 1:25 Mouse anti-CDH1 IgG2a Transduction Lab, BD Biosciences 61081 1:50 Mouse anti-CDH1 IgG1 ECM Biosciences (Versailles, KY) CM1681 1:50 Rabbit anti-GFP Polyclonal Novus Biologicals, LLC AB-4 1:500 Mouse anti-rat CD44 IgG2a Cedarlane CL044 1:50 Mouse OV6 IgG1 Kind gift from Dr. Stewart Sell (Wadsworth Center, Albany, NY) NA 1:50 Mouse anti-ACTA2 IgG2a Abcam Ab11003 1:100 Mouse anti-GFAP IgG1 Abcam Ab80842 1:400 Mouse anti-rat VCAM1 IgG1 Bio Legend (San Diego, CA) 200401 1:100 Rabbit anti-CD29/integrin B1 Monoclonal Epitomics (Burlingame, CA) 2288-1 1:50 Mouse anti-vimentin IgG1 Lab Vision Corporation (Fremont, CA) MS129-PO 1:50 Mouse anti-rat NT5E IgG1 BD Biosciences 551123 1:50 Open in a separate window NA, not applicable.

Techniques: Immunofluorescence, Microscopy, Expressing, Marker, Cell Culture